MUC1-C oncoprotein suppresses reactive oxygen species–induced terminal differentiation of acute myelogenous leukemia cells

• Published in: Blood 2011-05, Vol.117 (18), p.4863-4870
• Main Authors: Yin, Li, Wu, Zekui, Avigan, David, Rosenblatt, Jacalyn, Stone, Richard, Kharbanda, Surender, Kufe, Donald
• Format: Article
• Language: English
• Subjects: Acetylcysteine - pharmacology; Amino Acid Sequence; Apoptosis - drug effects; Biological and medical sciences; Cell Differentiation - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Hematologic and hematopoietic diseases; Humans; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - metabolism; Leukemia, Myeloid, Acute - pathology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Mucin-1 - chemistry; Mucin-1 - metabolism; Myeloid Neoplasia; Necrosis; Oligopeptides - chemistry; Oligopeptides - pharmacology; Oxidation-Reduction; Oxidative Stress; Phenotype; Protein Structure, Tertiary; Reactive Oxygen Species - metabolism
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2010-10-296632

Acute myeloid leukemia (AML) cells are characterized by unlimited self-renewal and an impaired capacity to undergo terminal differentiation. The MUC1 oncoprotein is aberrantly expressed in AML cells; however, there has been no evidence for involvement of MUC1 in myeloid leukemogenesis. Cell-penetrating peptide inhibitors of the MUC1-C subunit block its oligomerization and thereby oncogenic function. The present results demonstrate that treatment of human MOLM-14 and MV4-11 AML cells with these inhibitors is associated with arrest of growth, induction of late apoptosis/necrosis, and loss of self-renewal capacity. Similar results were obtained with primary blasts from patients with AML. Inhibition of MUC1-C was associated with increases in reactive oxygen species (ROS) and depletion of glutathione. Increases in ROS have been linked to induction of hematopoietic cell differentiation along the myeloid lineage. In this regard, inhibition of MUC1-C was associated with induction of a terminally differentiated myeloid phenotype in AML cell lines and primary blasts by an ROS-dependent mechanism. These findings indicate that MUC1-C function is of importance to AML cell self-renewal and that inhibition of MUC1-C represents a potential therapeutic approach to induce terminal differentiation of AML cells.