A new rRNA processing mutant of Saccharomyces cerevisiae

We have identified from a collection of temperature sensitive yeast mytants strains which fail to process rRNA normally. Characterization of one such mutant is reported here. This strain accumulates increased amounts of the 35S primary transcript, ‘24S’ molecules extending from the transcription sta...

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Bibliographic Details
Published in:Nucleic acids research 1992-01, Vol.20 (2), p.295-301
Main Authors: Lindahl, Lasse, Archer, Richard H., Zengel, Janice M.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Blotting, Northern
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Molecular Sequence Data
Mutation - genetics
RNA Precursors - genetics
RNA Precursors - metabolism
RNA Processing, Post-Transcriptional - genetics
RNA, Fungal - genetics
RNA, Fungal - metabolism
RNA, Ribosomal, 5.8S - genetics
RNA, Ribosomal, 5.8S - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Temperature
Thallophyta, bryophyta
Vegetals
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Summary:We have identified from a collection of temperature sensitive yeast mytants strains which fail to process rRNA normally. Characterization of one such mutant is reported here. This strain accumulates increased amounts of the 35S primary transcript, ‘24S’ molecules extending from the transcription start site to the 5.8S region, and two classes of 5.8S rRNA with 5' extensions of 7 and 149 bases, respectively. We show that this pleiotropic change in the rRNA processing pattern is dye to a single mutation. Possoble models for the function of the mutated gene are discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.2.295