Anti-Phospholipase A2 Receptor Antibody in Membranous Nephropathy

The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20...

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Bibliographic Details
Published in:Journal of the American Society of Nephrology 2011-06, Vol.22 (6), p.1137-1143
Main Authors: WEISONG QIN, BECK, Laurence H, CAIHONG ZENG, ZHAOHONG CHEN, SHIJUN LI, KE ZUO, SALANT, David J, ZHIHONG LIU
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Anti-Idiotypic - blood
Biological and medical sciences
Biomarkers - blood
Blotting, Western
Case-Control Studies
China
Clinical Research
Female
Glomerulonephritis
Glomerulonephritis, Membranous - diagnosis
Glomerulonephritis, Membranous - ethnology
Glomerulonephritis, Membranous - immunology
Humans
Male
Medical sciences
Middle Aged
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Receptors, Phospholipase A2 - immunology
Sensitivity and Specificity
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Summary:The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20 with lupus-associated MN, 16 with hepatitis B (HBV)-associated MN, and 10 with tumor-associated MN. Among patients with idiopathic MN, 49 (82%) had detectable anti-PLA2R autoantibodies using a Western blot assay; an assay with greater sensitivity detected very low titers of anti-PLA2R in 10 of the remaining 11 patients. Using the standard assay, we detected anti-PLA2R antibody in only 1 patient with lupus, 1 with HBV, and 3 with cancer, producing an overall specificity of 89% in this cohort limited to patients with secondary MN. The enhanced assay detected low titers of anti-PLA2R in only 2 additional samples of HBV-associated MN. In summary, these results suggest that PLA2R is a major target antigen in Chinese idiopathic MN and that detection of anti-PLA2R is a sensitive test for idiopathic MN.
ISSN:1046-6673
1533-3450
DOI:10.1681/asn.2010090967