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Anti-Phospholipase A2 Receptor Antibody in Membranous Nephropathy

The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20...

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Published in:	Journal of the American Society of Nephrology 2011-06, Vol.22 (6), p.1137-1143
Main Authors:	WEISONG QIN, BECK, Laurence H, CAIHONG ZENG, ZHAOHONG CHEN, SHIJUN LI, KE ZUO, SALANT, David J, ZHIHONG LIU
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Language:	English
Subjects:	Adult Aged Antibodies, Anti-Idiotypic - blood Biological and medical sciences Biomarkers - blood Blotting, Western Case-Control Studies China Clinical Research Female Glomerulonephritis Glomerulonephritis, Membranous - diagnosis Glomerulonephritis, Membranous - ethnology Glomerulonephritis, Membranous - immunology Humans Male Medical sciences Middle Aged Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Receptors, Phospholipase A2 - immunology Sensitivity and Specificity
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creator	WEISONG QIN BECK, Laurence H CAIHONG ZENG ZHAOHONG CHEN SHIJUN LI KE ZUO SALANT, David J ZHIHONG LIU
description	The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20 with lupus-associated MN, 16 with hepatitis B (HBV)-associated MN, and 10 with tumor-associated MN. Among patients with idiopathic MN, 49 (82%) had detectable anti-PLA2R autoantibodies using a Western blot assay; an assay with greater sensitivity detected very low titers of anti-PLA2R in 10 of the remaining 11 patients. Using the standard assay, we detected anti-PLA2R antibody in only 1 patient with lupus, 1 with HBV, and 3 with cancer, producing an overall specificity of 89% in this cohort limited to patients with secondary MN. The enhanced assay detected low titers of anti-PLA2R in only 2 additional samples of HBV-associated MN. In summary, these results suggest that PLA2R is a major target antigen in Chinese idiopathic MN and that detection of anti-PLA2R is a sensitive test for idiopathic MN.
doi_str_mv	10.1681/asn.2010090967
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In summary, these results suggest that PLA2R is a major target antigen in Chinese idiopathic MN and that detection of anti-PLA2R is a sensitive test for idiopathic MN.</description><identifier>ISSN: 1046-6673</identifier><identifier>EISSN: 1533-3450</identifier><identifier>DOI: 10.1681/asn.2010090967</identifier><identifier>PMID: 21566055</identifier><identifier>CODEN: JASNEU</identifier><language>eng</language><publisher>Washington, DC: American Society of Nephrology</publisher><subject>Adult ; Aged ; Antibodies, Anti-Idiotypic - blood ; Biological and medical sciences ; Biomarkers - blood ; Blotting, Western ; Case-Control Studies ; China ; Clinical Research ; Female ; Glomerulonephritis ; Glomerulonephritis, Membranous - diagnosis ; Glomerulonephritis, Membranous - ethnology ; Glomerulonephritis, Membranous - immunology ; Humans ; Male ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. 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Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20 with lupus-associated MN, 16 with hepatitis B (HBV)-associated MN, and 10 with tumor-associated MN. Among patients with idiopathic MN, 49 (82%) had detectable anti-PLA2R autoantibodies using a Western blot assay; an assay with greater sensitivity detected very low titers of anti-PLA2R in 10 of the remaining 11 patients. Using the standard assay, we detected anti-PLA2R antibody in only 1 patient with lupus, 1 with HBV, and 3 with cancer, producing an overall specificity of 89% in this cohort limited to patients with secondary MN. The enhanced assay detected low titers of anti-PLA2R in only 2 additional samples of HBV-associated MN. In summary, these results suggest that PLA2R is a major target antigen in Chinese idiopathic MN and that detection of anti-PLA2R is a sensitive test for idiopathic MN.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Anti-Idiotypic - blood</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>China</subject><subject>Clinical Research</subject><subject>Female</subject><subject>Glomerulonephritis</subject><subject>Glomerulonephritis, Membranous - diagnosis</subject><subject>Glomerulonephritis, Membranous - ethnology</subject><subject>Glomerulonephritis, Membranous - immunology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephropathies. Renovascular diseases. 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Urinary tract diseases</topic><topic>Nephropathies. Renovascular diseases. Renal failure</topic><topic>Receptors, Phospholipase A2 - immunology</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WEISONG QIN</creatorcontrib><creatorcontrib>BECK, Laurence H</creatorcontrib><creatorcontrib>CAIHONG ZENG</creatorcontrib><creatorcontrib>ZHAOHONG CHEN</creatorcontrib><creatorcontrib>SHIJUN LI</creatorcontrib><creatorcontrib>KE ZUO</creatorcontrib><creatorcontrib>SALANT, David J</creatorcontrib><creatorcontrib>ZHIHONG LIU</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Society of Nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WEISONG QIN</au><au>BECK, Laurence H</au><au>CAIHONG ZENG</au><au>ZHAOHONG CHEN</au><au>SHIJUN LI</au><au>KE ZUO</au><au>SALANT, David J</au><au>ZHIHONG LIU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-Phospholipase A2 Receptor Antibody in Membranous Nephropathy</atitle><jtitle>Journal of the American Society of Nephrology</jtitle><addtitle>J Am Soc Nephrol</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>22</volume><issue>6</issue><spage>1137</spage><epage>1143</epage><pages>1137-1143</pages><issn>1046-6673</issn><eissn>1533-3450</eissn><coden>JASNEU</coden><abstract>The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R is unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody in the serum of 60 patients with idiopathic MN, 20 with lupus-associated MN, 16 with hepatitis B (HBV)-associated MN, and 10 with tumor-associated MN. Among patients with idiopathic MN, 49 (82%) had detectable anti-PLA2R autoantibodies using a Western blot assay; an assay with greater sensitivity detected very low titers of anti-PLA2R in 10 of the remaining 11 patients. Using the standard assay, we detected anti-PLA2R antibody in only 1 patient with lupus, 1 with HBV, and 3 with cancer, producing an overall specificity of 89% in this cohort limited to patients with secondary MN. The enhanced assay detected low titers of anti-PLA2R in only 2 additional samples of HBV-associated MN. 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subjects	Adult Aged Antibodies, Anti-Idiotypic - blood Biological and medical sciences Biomarkers - blood Blotting, Western Case-Control Studies China Clinical Research Female Glomerulonephritis Glomerulonephritis, Membranous - diagnosis Glomerulonephritis, Membranous - ethnology Glomerulonephritis, Membranous - immunology Humans Male Medical sciences Middle Aged Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Receptors, Phospholipase A2 - immunology Sensitivity and Specificity
title	Anti-Phospholipase A2 Receptor Antibody in Membranous Nephropathy
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