Volunteer Challenge With Enterotoxigenic "Escherichia coli" That Express Intestinal Colonization Factor Fimbriae CS17 and CS19

Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dosedependent eff...

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Bibliographic Details
Published in:The Journal of infectious diseases 2011-07, Vol.204 (1), p.60-64
Main Authors: McKenzie, Robin, Porter, Chad K., Cantrell, Joyce A., DeNearing, Barbara, O'Dowd, Aisling, Grahek, Shannon L., Sincock, Stephanie A., Woods, Colleen, Sebeny, Peter, Sack, David A., Tribble, David R., Bourgeois, A. Louis, Savarino, Stephen J.
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - biosynthesis
Adhesins, Bacterial - immunology
Adolescent
Adult
Animal models
Antibodies, Bacterial - blood
BACTERIA
Bacterial Toxins - immunology
Bacteriology
Biological and medical sciences
Diarrhea
Diarrhea - microbiology
Diarrhea - pathology
Dosage
Enterotoxigenic Escherichia coli - growth & development
Enterotoxigenic Escherichia coli - pathogenicity
Enterotoxins - immunology
Epidemiology
Escherichia coli
Escherichia coli Infections - microbiology
Escherichia coli Infections - pathology
Escherichia coli Proteins - biosynthesis
Escherichia coli Proteins - immunology
Female
Fimbriae
Fundamental and applied biological sciences. Psychology
Human Experimentation
Humans
Infectious diseases
Major and Brief Reports
Male
Medical research
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Toxins
Vaccination
Virulence Factors - biosynthesis
Virulence Factors - immunology
Volunteerism
Young Adult
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Summary:Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dosedependent effect, with the upper dose (6 X 10⁹ organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 X 10⁹ organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jir220