Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

WASP and SCAR homologue (WASH) is a recently identified and evolutionarily conserved regulator of actin polymerization. In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material. A related process, the expulsion of the l...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 2011-05, Vol.193 (5), p.831-839
Main Authors: Carnell, Michael, Zech, Tobias, Calaminus, Simon D, Ura, Seiji, Hagedorn, Monica, Johnston, Simon A, May, Robin C, Soldati, Thierry, Machesky, Laura M, Insall, Robert H
Format: Article
Language:English
Subjects:
Actins - chemistry
Actins - metabolism
Adenosine triphosphatase
Bacteria
Cells
Cryptococcus neoformans - metabolism
Cryptococcus neoformans - pathogenicity
Dictyostelium - cytology
Dictyostelium - metabolism
Exocytosis
Lysosomes - metabolism
Mammals
Microfilament Proteins - metabolism
Mutation
Polymerization
Proteins
Vacuolar Proton-Translocating ATPases - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:WASP and SCAR homologue (WASH) is a recently identified and evolutionarily conserved regulator of actin polymerization. In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible material. A related process, the expulsion of the lethal endosomal pathogen Cryptococcus neoformans from mammalian macrophages, also uses WASH-coated vesicles, and cells expressing dominant negative WASH mutants inefficiently expel C. neoformans. D. discoideum WASH causes filamentous actin (F-actin) patches to form on lysosomes, leading to the removal of vacuolar adenosine triphosphatase (V-ATPase) and the neutralization of lysosomes to form postlysosomes. Without WASH, no patches or coats are formed, neutral postlysosomes are not seen, and indigestible material such as dextran is not exocytosed. Similar results occur when actin polymerization is blocked with latrunculin. V-ATPases are known to bind avidly to F-actin. Our data imply a new mechanism, actin-mediated sorting, in which WASH and the Arp2/3 complex polymerize actin on vesicles to drive the separation and recycling of proteins such as the V-ATPase.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.201009119