Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis

Mammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation a...

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Bibliographic Details
Published in:The Journal of cell biology 2011-05, Vol.193 (5), p.917-933
Main Authors: Hurtado, Lidia, Caballero, Cristina, Gavilan, Maria P, Cardenas, Jesus, Bornens, Michel, Rios, Rosa M
Format: Article
Language:English
Subjects:
A Kinase Anchor Proteins - metabolism
Binding sites
Cell adhesion & migration
Cell Movement
Cells
Cells, Cultured
Centrosome - metabolism
Cilia - metabolism
Cytoskeletal Proteins - metabolism
Epithelial Cells - metabolism
Genotype & phenotype
Golgi Apparatus - metabolism
Humans
Mammals
Microtubules - metabolism
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Summary:Mammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation at the Golgi without interfering with centrosomal activity. These two fragments had, however, strikingly different effects on both Golgi apparatus (GA) integrity and positioning, whereas the short fragment induced GA circularization and ribbon fragmentation, the large construct that encompasses an additional p150glued/MT-binding domain induced separation of the Golgi ribbon from the centrosome. These distinct phenotypes arose by specific interference of each fragment with either Golgi-dependent or centrosome-dependent stages of Golgi assembly. We could thus demonstrate that breaking the polarity axis by perturbing GA positioning has a more dramatic effect on directional cell migration than disrupting the Golgi ribbon. Both features, however, were required for ciliogenesis. We thus identified AKAP450 as a key determinant of pericentrosomal Golgi ribbon integrity, positioning, and function in mammalian cells.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.201011014