Capturing diversity of marine heterotrophic protists: one cell at a time

Recent applications of culture-independent, molecular methods have revealed unexpectedly high diversity in a variety of functional and phylogenetic groups of microorganisms in the ocean. However, none of the existing research tools are free from significant limitations, such as PCR and cloning biase...

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Bibliographic Details
Published in:The ISME Journal 2011-04, Vol.5 (4), p.674-684
Main Authors: Heywood, Jane L, Sieracki, Michael E, Bellows, Wendy, Poulton, Nicole J, Stepanauskas, Ramunas
Format: Article
Language:English
Subjects:
631/158/670
631/1647/514
631/326/2565
Aquatic Organisms - classification
Aquatic Organisms - genetics
Aquatic Organisms - isolation & purification
Biodiversity
Biomedical and Life Sciences
Cloning
Community composition
Dinophyceae
Ecology
Environment
Eukaryota - classification
Eukaryota - genetics
Eukaryota - isolation & purification
Evolutionary Biology
Flow Cytometry
Gene Library
Life Sciences
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microorganisms
Original
original-article
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 18S - genetics
Seawater
Sequence Analysis, DNA
Stramenopiles
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Summary:Recent applications of culture-independent, molecular methods have revealed unexpectedly high diversity in a variety of functional and phylogenetic groups of microorganisms in the ocean. However, none of the existing research tools are free from significant limitations, such as PCR and cloning biases, low phylogenetic resolution and others. Here, we employed novel, single-cell sequencing techniques to assess the composition of small (
ISSN:1751-7362
1751-7370
DOI:10.1038/ismej.2010.155