Optical mapping of BAC clones from the human Y chromosome DAZ locus

The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y...

Full description

Saved in:
Bibliographic Details
Published in:Genome research 2000-09, Vol.10 (9), p.1421-1429
Main Authors: Giacalone, J, Delobette, S, Gibaja, V, Ni, L, Skiadas, Y, Qi, R, Edington, J, Lai, Z, Gebauer, D, Zhao, H, Anantharaman, T, Mishra, B, Brown, L G, Saxena, R, Page, D C, Schwartz, D C
Format: Article
Language:English
Subjects:
Chromosome Mapping - instrumentation
Chromosome Mapping - methods
Chromosomes, Bacterial - genetics
Cloning, Molecular - methods
Contig Mapping
Deleted in Azoospermia 1 Protein
Deoxyribonucleases, Type II Site-Specific - analysis
Genetic Markers - genetics
Humans
Methods
Mutagenesis, Insertional
Restriction Mapping
RNA-Binding Proteins - analysis
RNA-Binding Proteins - genetics
Y Chromosome - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.112100