Methylation analysis by means of PCR SSCP: application to clonality studies

We have developed a rapid procedure for determining the differing methylation status, on maternally and paternally derived alleles, of particular CpG dinucleotide repeats. Unlike a previously published technique of van Kamp et al. this method does not require the use of restriction endonucleases to...

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Bibliographic Details
Published in:Nucleic acids research 1993-09, Vol.21 (19), p.4655-4655
Main Authors: Corcoran, Martin M., Ibbotson, Rachel E., Lu, Wei Z., Chapman, Robert M., Oscier, David G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Diverse techniques
Electrophoresis, Polyacrylamide Gel - methods
Fundamental and applied biological sciences. Psychology
Humans
Methylation
Molecular and cellular biology
Nucleic Acid Denaturation
Phosphotransferases (Alcohol Group Acceptor) - genetics
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Online Access:Get full text
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Summary:We have developed a rapid procedure for determining the differing methylation status, on maternally and paternally derived alleles, of particular CpG dinucleotide repeats. Unlike a previously published technique of van Kamp et al. this method does not require the use of restriction endonucleases to differentiate between the two alleles on the basis of the presence or absence of a polymorphic cutting site. We have employed a modification of this technique based on PCR and the technique of Single Stranded Conformational Polymorphism (SSCP) analysis of Orita et al. to determine the methylation status of two CpG dinucleotide sites in the sequence of the chromosome X-linked PGK-1 gene.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/21.19.4655