Synthesis and Bioconjugation of 2 and 3 nm-Diameter Gold Nanoparticles

By adjustment of solvent conditions for synthesis, virtually monodisperse 4-mercaptobenzoic acid (p-MBA) monolayer-protected gold nanoparticles, 2 and 3 nm in diameter, were obtained. Large single crystals of the 2 nm particles could be grown from the reaction mixture. Uniformity was also demonstrat...

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Bibliographic Details
Published in:Bioconjugate chemistry 2010-02, Vol.21 (2), p.214-218
Main Authors: Ackerson, Christopher J, Jadzinsky, Pablo D, Sexton, Jonathan Z, Bushnell, David A, Kornberg, Roger D
Format: Article
Language:English
Subjects:
Benzoates - chemistry
Crystals
Cysteine
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrons
Gold - chemistry
Metal Nanoparticles - chemistry
Microscopy, Electron
Mutation
Nanoparticles
Particle Size
Proteins
Proteins - chemistry
Proteins - genetics
Scanning electron microscopy
Solvents
Sulfhydryl Compounds - chemistry
Thionucleotides - chemistry
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Summary:By adjustment of solvent conditions for synthesis, virtually monodisperse 4-mercaptobenzoic acid (p-MBA) monolayer-protected gold nanoparticles, 2 and 3 nm in diameter, were obtained. Large single crystals of the 2 nm particles could be grown from the reaction mixture. Uniformity was also demonstrated by the formation of two-dimensional arrays and by quantitative high-angle annular dark-field scanning transmission electron microscopy. The 2 and 3 nm particles were spontaneously reactive for conjugation with proteins and DNA, and further reaction could be prevented by repassivation with glutathione. Conjugates with antibody Fc fragment could be used to identify TAP-tagged proteins of interest in electron micrographs, through the binding of a pair of particles to the pair of protein A domains in the TAP tag.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc900135d