Pressure induced nucleus DNA fragmentation

Purpose The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H); N  = 15] aged 2–3 months were exposed into the pressure impulse lasting ~0.021 s and charact...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2011-04, Vol.28 (4), p.363-368
Main Authors: Grygoruk, Cezary, Sieczynski, Piotr, Modlinski, Jacek A., Gajda, Barbara, Greda, Pawel, Grad, Izabela, Pietrewicz, Piotr, Mrugacz, Grzegorz
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Blastocyst - cytology
Catheters
Cell Nucleus - metabolism
DNA Fragmentation
Embryology
Embryos
Female
Gynecology
Human Genetics
In Situ Nick-End Labeling
Male
Medicine
Medicine & Public Health
Mice
Mice, Inbred C57BL
Pregnancy
Pressure
Reproductive health
Reproductive Medicine
Technical Innovations
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Summary:Purpose The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H); N  = 15] aged 2–3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. Results The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p  
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-010-9525-1