Isolation and characterization of the human catalase gene

catalase is a tetrameric hemoprotein which degrades H2O2. Recombinant phage clones containing the human catalase gene have been isolated and characterized. The gene is 34kb long and is split into 13 exons. The precise size and location of the exons has been determined. In addition essentially full l...

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Bibliographic Details
Published in:Nucleic acids research 1986-07, Vol.14 (13), p.5321-5335
Main Authors: Quan, F., Korneluk, R.G., Tropak, M.B., Gravel, R.A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Catalase - genetics
Cloning, Molecular
DNA - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Humans
Molecular and cellular biology
Molecular genetics
Transcription, Genetic
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Summary:catalase is a tetrameric hemoprotein which degrades H2O2. Recombinant phage clones containing the human catalase gene have been isolated and characterized. The gene is 34kb long and is split into 13 exons. The precise size and location of the exons has been determined. In addition essentially full length catalase cDNA clones have been isolated and sequenced and used to tentatively identify the 5′-end of the gene. This assignment if correct, predicts that the region upstream of the gene does not contain a TATA box. This region is GC rich (67%) and contains several CCAAT and GGGCGG sequences which may form part of the promoter. Translation of the catalase mRNA appears to begin immediately upstream of the amino-terminal Ala residue of catalase.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.13.5321