Covalently linked complementary oligodeoxynucleotides (splinkers) as tools for molecular biology

Self-complementary primer linkers ("splinkers") specifically join to restricted DNA. These oligonucleotides consist of an inverted repeat sequence which forms a double stranded hairpin structure containing a known restriction site (RS). This built-in restriction site enables their specific...

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Bibliographic Details
Published in:Nucleic acids research 1986-09, Vol.14 (17), p.7131-7131
Main Authors: Krawetz, Stephen A., Kalisch, Bernd W., van de Sande, Johan H.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
DNA Replication
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Genetic Engineering - methods
Nucleic Acid Hybridization
Nucleic acids
nucleotide sequence
oligodeoxynucleotides
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Description
Summary:Self-complementary primer linkers ("splinkers") specifically join to restricted DNA. These oligonucleotides consist of an inverted repeat sequence which forms a double stranded hairpin structure containing a known restriction site (RS). This built-in restriction site enables their specific release from the restricted DNA fragment. They can be used as strand specific sequence primers for dideoxy sequencing of either cloned or genomic DNA.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.17.7131