An Intermediate Membrane Subfraction in Cyanobacteria Is Involved in an Assembly Network for Photosystem II Biogenesis

Early steps in the biogenesis of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803 are thought to occur in a specialized membrane fraction that is characterized by the specific accumulation of the PSII assembly factor PratA and its interaction partner pD1, the precursor of the D...

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Bibliographic Details
Published in:The Journal of biological chemistry 2011-06, Vol.286 (24), p.21944-21951
Main Authors: Rengstl, Birgit, Oster, Ulrike, Stengel, Anna, Nickelsen, Jörg
Format: Article
Language:English
Subjects:
Cell Membrane - metabolism
Chlorophyllide a
Chlorophyllides - chemistry
Cyanobacteria - metabolism
Immunoblotting
Immunoprecipitation
Lipids - chemistry
Membrane Biogenesis
Membrane Biology
Models, Biological
Mutation
Photosynthesis
Photosynthesis - genetics
Photosynthetic Pigments
Photosystem II
Photosystem II Protein Complex - metabolism
Pigmentation
Protein Assembly
Protein Conformation
Protein Targeting
Synechocystis
Synechocystis - metabolism
Thylakoids - metabolism
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Summary:Early steps in the biogenesis of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803 are thought to occur in a specialized membrane fraction that is characterized by the specific accumulation of the PSII assembly factor PratA and its interaction partner pD1, the precursor of the D1 protein of PSII. Here, we report the molecular characterization of this membrane fraction, called the PratA-defined membrane (PDM), with regard to its lipid and pigment composition and its association with PSII assembly factors, including YCF48, Slr1471, Sll0933, and Pitt. We demonstrate that YCF48 and Slr1471 are present and that the chlorophyll precursor chlorophyllide a accumulates in the PDM. Analysis of PDMs from various mutant lines suggests a central role for PratA in the spatial organization of PSII biogenesis. Moreover, quantitative immunoblot analyses revealed a network of interdependences between several PSII assembly factors and chlorophyll synthesis. In addition, formation of complexes containing both YCF48 and Sll0933 was substantiated by co-immunoprecipitation experiments. The findings are integrated into a refined model for PSII biogenesis in Synechocystis 6803.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M111.237867