Usefulness of Polymerase Chain Reaction to Supplement Field Microscopy in a Pre-Selected Population with a High Probability of Malaria Infections

This study determines the use of nested PCR as a diagnostic tool to supplement field microscopy in symptomatic individuals suspected of being positive for malaria, and it explores its role in active case detection to identify asymptomatic parasite carriers. In symptomatic individuals, compared with...

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Published in:The American journal of tropical medicine and hygiene 2011-07, Vol.85 (1), p.6-11
Main Authors: KUMUDUNAYANA, W. M, GUNASEKERA, T. De A. W, ABEYASINGHE, Rabindra R, PREMAWANSA, Sunil, DEEPIKA FERNANDO, S
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Carrier State
Female
Human protozoal diseases
Humans
Infectious diseases
Malaria
Malaria - diagnosis
Malaria - transmission
Male
Medical sciences
Parasitic diseases
Polymerase Chain Reaction - methods
Probability
Protozoal diseases
Sri Lanka
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container_title The American journal of tropical medicine and hygiene
container_volume 85
creator KUMUDUNAYANA, W. M
GUNASEKERA, T. De A. W
ABEYASINGHE, Rabindra R
PREMAWANSA, Sunil
DEEPIKA FERNANDO, S
description This study determines the use of nested PCR as a diagnostic tool to supplement field microscopy in symptomatic individuals suspected of being positive for malaria, and it explores its role in active case detection to identify asymptomatic parasite carriers. In symptomatic individuals, compared with PCR, microscopy had a sensitivity of 86.6% (95% confidence interval [CI] = 77.8-92.4) and specificity of 100% (95% CI = 96.9-100). During active case detection, two asymptomatic persons were diagnosed as having vivax malaria by polymerase chain reaction (PCR) but not microscopy. Currently, PCR is being carried out in Sri Lanka only for population surveys to estimate the hidden reservoir of malaria. Based on the results of this study and because of cost considerations, pooled PCR will be used in the future to screen samples from clinically suspected foci to increase the proportion of malaria cases detected. This strategy will assist the success of the malaria elimination program in Sri Lanka.
doi_str_mv 10.4269/ajtmh.2011.10-0337
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ispartof The American journal of tropical medicine and hygiene, 2011-07, Vol.85 (1), p.6-11
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1476-1645
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subjects Adult
Biological and medical sciences
Carrier State
Female
Human protozoal diseases
Humans
Infectious diseases
Malaria
Malaria - diagnosis
Malaria - transmission
Male
Medical sciences
Parasitic diseases
Polymerase Chain Reaction - methods
Probability
Protozoal diseases
Sri Lanka
title Usefulness of Polymerase Chain Reaction to Supplement Field Microscopy in a Pre-Selected Population with a High Probability of Malaria Infections
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