Distribution and Phylogenetic Comparisons of a Novel Mosquito Flavivirus Sequence Present in Culex tarsalis Mosquitoes from Western Canada with Viruses Isolated in California and Colorado

In a previous study, a new flavivirus genome sequence was identified in Culex tarsalis mosquitoes obtained in Alberta, Canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. Nonstructural protein 5-encoding sequences amplified from Cx. tarsal...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2011-07, Vol.85 (1), p.162-168
Main Authors: TYLER, Shaun, BOLLING, Bethany G, BLAIR, Carol D, BRAULT, Aaron C, PABBARAJU, Kanti, ARMIJOS, M. Veronica, CLARK, David C, CALISHER, Charles H, DREBOT, Michael A
Format: Article
Language:English
Subjects:
Alberta
Animals
Base Sequence
Biological and medical sciences
California
Colorado
Culicidae - virology
DNA Primers
Flavivirus - classification
Flavivirus - genetics
Flavivirus - isolation & purification
Infectious diseases
Medical sciences
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
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Summary:In a previous study, a new flavivirus genome sequence was identified in Culex tarsalis mosquitoes obtained in Alberta, Canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. Nonstructural protein 5-encoding sequences amplified from Cx. tarsalis pools from western Canada have shown a high similarity to genome sequences of novel flaviviruses isolated from mosquitoes in California and Colorado. Despite wide distribution of this virus, designated Calbertado virus, strains demonstrate a high degree of nonstructural protein 5 nucleotide (> 90%) and amino acid (> 97%) identity. The ecology and geographic range of Calbertado virus warrants further study because it may potentially influence transmission of mosquito-borne flaviviruses, including important human pathogens such as West Nile and Saint Louis encephalitis viruses.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2011.10-0469