A novel method for the isolation of tissue-specific genes

Subtraction cloning has become a popular method for isolating tissue specific genes. One of the most common problems with subtraction techniques in that specific mRNAs or cDNAs from the target tissue are subtracted away by common sequences (e.g. gene families) or repetitive elements (e.g. Alu repeti...

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Bibliographic Details
Published in:Nucleic acids research 1992-07, Vol.20 (13), p.3528-3528
Main Authors: RODRIGUEZ, I. R, CHADER, G. J
Format: Article
Language:English
Subjects:
antisense
Biological and medical sciences
cDNA
Cloning, Molecular
Diverse techniques
DNA Probes - biosynthesis
DNA Probes - genetics
DNA Probes - metabolism
DNA, Antisense - biosynthesis
DNA, Antisense - genetics
DNA, Antisense - metabolism
Fundamental and applied biological sciences. Psychology
genes
Humans
isolation
Molecular and cellular biology
Oligodeoxyribonucleotides - genetics
Oligodeoxyribonucleotides - metabolism
probes
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
specificity
tissues
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Summary:Subtraction cloning has become a popular method for isolating tissue specific genes. One of the most common problems with subtraction techniques in that specific mRNAs or cDNAs from the target tissue are subtracted away by common sequences (e.g. gene families) or repetitive elements (e.g. Alu repetitive elements) leaving little or no product to clone or use as a probe. We have devised a new subtraction method for the generation of tissue-specific probes which avoids these problems.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.13.3528