COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis

To control organ shape, plant cells expand differentially. The organization of the cellulose microfibrils in the cell wall is a key determinant of differential expansion. Mutations in the COBRA (COB) gene of Arabidopsis, known to affect the orientation of cell expansion in the root, are reported her...

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Bibliographic Details
Published in:Genes & development 2001-05, Vol.15 (9), p.1115-1127
Main Authors: Schindelman, G, Morikami, A, Jung, J, Baskin, T I, Carpita, N C, Derbyshire, P, McCann, M C, Benfey, P N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Apoproteins
Arabidopsis
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins
Base Sequence
cell elongation
Cell Membrane - metabolism
Cell Polarity - physiology
Cellulose - metabolism
Chromosome Mapping
Cloning, Molecular
COBRA (COB) gene
Cytochrome b Group
Cytochromes b
Gene Expression Regulation, Plant
Glycosylphosphatidylinositols - metabolism
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Molecular Sequence Data
Mutation
Plant Roots - cytology
Research Paper
RNA, Plant - metabolism
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Summary:To control organ shape, plant cells expand differentially. The organization of the cellulose microfibrils in the cell wall is a key determinant of differential expansion. Mutations in the COBRA (COB) gene of Arabidopsis, known to affect the orientation of cell expansion in the root, are reported here to reduce the amount of crystalline cellulose in cell walls in the root growth zone. The COB gene, identified by map-based cloning, contains a sequence motif found in proteins that are anchored to the extracellular surface of the plasma membrane through a glycosylphosphatidylinositol (GPI) linkage. In animal cells, this lipid linkage is known to confer polar localization to proteins. The COB protein was detected predominately on the longitudinal sides of root cells in the zone of rapid elongation. Moreover, COB RNA levels are dramatically upregulated in cells entering the zone of rapid elongation. Based on these results, models are proposed for the role of COB as a regulator of oriented cell expansion.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.879101