Maximizing target protein ablation by integration of RNAi and protein knockout

Dear Editor, We have developed a method that achieves a faster and more thorough ablation of cellular proteins by integrating RNAi and protein knockout techniques （Figure 1A）. While this combination approach is generally applicable to any target protein, it is ideal for those that cannot be sufficie...

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Bibliographic Details
Published in:Cell research 2011-07, Vol.21 (7), p.1152-1154
Main Authors: Hannah, Jeffrey, Zhou, Pengbo
Format: Article
Language:English
Subjects:
631/1647/1407/505
631/80
Animals
Biomedical and Life Sciences
Cell Biology
Cell Line
Gene Knockdown Techniques - methods
Humans
Letter to the Editor
Life Sciences
Proteins - genetics
RNA Interference
RNA, Small Interfering - genetics
RNAi技术
基因敲除
生物合成
细胞
蛋白质结合
融合蛋白
长寿命
靶蛋白
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Summary:Dear Editor, We have developed a method that achieves a faster and more thorough ablation of cellular proteins by integrating RNAi and protein knockout techniques （Figure 1A）. While this combination approach is generally applicable to any target protein, it is ideal for those that cannot be sufficiently eradicated by RNAi and for long-lived proteins that endure well after nascent protein biosynthesis has been halted.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2011.89