An Amino Acid-based Amphoteric Liposomal Delivery System for Systemic Administration of siRNA

• Published in: Molecular therapy 2011-06, Vol.19 (6), p.1141-1151
• Main Authors: Adami, Roger C, Seth, Shaguna, Harvie, Pierrot, Johns, Rachel, Fam, Renata, Fosnaugh, Kathy, Zhu, Tianying, Farber, Ken, McCutcheon, Michael, Goodman, Thomas T, Liu, Yan, Chen, Yan, Kwang, Erin, Templin, Michael V, Severson, Greg, Brown, Tod, Vaish, Narendra, Chen, Feng, Charmley, Patrick, Polisky, Barry, Houston, Michael E
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - chemistry; Animals; Carbon; Cholesterol; Female; Genes; Hep G2 Cells; Humans; Lipids; Liposomes - chemistry; Liver; Mice; Mice, Inbred BALB C; Original; Phase transitions; RNA, Small Interfering - genetics
• ISSN: 1525-0016; 1525-0024
• DOI: 10.1038/mt.2011.56

We demonstrate a systematic and rational approach to create a library of natural and modified, dialkylated amino acids based upon arginine for development of an efficient small interfering RNA (siRNA) delivery system. These amino acids, designated DiLA2 compounds, in conjunction with other components, demonstrate unique properties for assembly into monodisperse, 100-nm small liposomal particles containing siRNA. We show that DiLA2-based liposomes undergo a pH-dependent phase transition to an inverted hexagonal phase facilitating efficient siRNA release from endosomes to the cytosol. Using an arginine-based DiLA2, cationic liposomes were prepared that provide high in vivo siRNA delivery efficiency and are well-tolerated in both cell and animal models. DiLA2-based liposomes demonstrate a linear dose–response with an ED50 of 0.1 mg/kg against liver-specific target genes in BALB/c mice.