Plasma lipases and lipid transfer proteins increase phospholipid but not free cholesterol transfer from lipid emulsion to high density lipoproteins

Plasma lipases and lipid transfer proteins are involved in the generation and speciation of high density lipoproteins. In this study we have examined the influence of plasma lipases and lipid transfer protein activities on the transfer of free cholesterol (FC) and phospholipids (PL) from lipid emuls...

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Bibliographic Details
Published in:BMC biochemistry 2001-02, Vol.2 (1), p.1-1, Article 1
Main Authors: Nunes, V S, Quintão, E C, Cazita, P M, Harada, L M, de Faria, E C, Oliveira, H C
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Carrier Proteins - metabolism
Cholesterol - analysis
Cholesterol - metabolism
Cholesterol Ester Transfer Proteins
Emulsions
Female
Glycoproteins
Humans
Lipase - blood
Lipase - metabolism
Lipid Metabolism
Lipids - chemistry
Lipoproteins - chemistry
Lipoproteins, HDL - chemistry
Male
Mice
Phospholipids - analysis
Phospholipids - metabolism
Rats
Rats, Wistar
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Summary:Plasma lipases and lipid transfer proteins are involved in the generation and speciation of high density lipoproteins. In this study we have examined the influence of plasma lipases and lipid transfer protein activities on the transfer of free cholesterol (FC) and phospholipids (PL) from lipid emulsion to human, rat and mouse lipoproteins. The effect of the lipases was verified by incubation of labeled (3H-FC,14C-PL) triglyceride rich emulsion with human plasma (control, post-heparin and post-heparin plus lipase inhibitor), rat plasma (control and post-heparin) and by the injection of the labeled lipid emulsion into control and heparinized functionally hepatectomized rats. In vitro, the lipase enriched plasma stimulated significantly the transfer of 14C-PL from emulsion to high density lipoprotein (p
ISSN:1471-2091
1471-2091
DOI:10.1186/1471-2091-2-1