Screening Antitumor Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds

Psoralea corylifolia L. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds of Psoralea corylifolia L. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from m...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2011-01, Vol.2011 (2011), p.1-7
Main Authors: Wang, Yi, Hong, Chengtao, Zhou, Chenguang, Xu, Dongmei, Qu, Hai-bin
Format: Article
Language:English
Subjects:
Alternative medicine
Amino acids
Annexin V
Antitumor activity
Antitumor agents
Apoptosis
Bioactive compounds
Breast cancer
Cancer
Chinese medicine
Chromatography
Cytotoxicity
Drug dosages
Flow cytometry
High-performance liquid chromatography
Liquid chromatography
Medical screening
Original
Pharmaceutical sciences
Psoralea corylifolia
Psoralen
Seeds
Tumor cell lines
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Summary:Psoralea corylifolia L. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds of Psoralea corylifolia L. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from methanol extraction of P. corylifolia L. were obtained. Bioactivities of these fractions were evaluated by the cytotoxicity on KB, KBv200, K562, K562/ADM cancer cells with MTT assay. Major components in the active fraction were identified by HPLC/MSn. Fraction IV significantly inhibits the growth of cancer cells in a dose-dependent manner. The IC50 values were 21.6, 24.4, 10.0 and 26.9, respectively. Psoralen and isopsoralen, isolated from fraction IV, were subject to bioactive assay and presented a dose-dependent anticancer activity in four cancer cell lines (KB, KBv200, K562 and K562/ADM). The IC50 values of psoralen were 88.1, 86.6, 24.4 and 62.6, which of isopsoralen were 61.9, 49.4, 49.6 and 72.0, respectively. Apoptosis of tumor cell significantly increased after treated with psoralen and isopsoralen. Induction of apoptotic activity was confirmed by flow cytometry after staining with Annexin V/PI. These results suggested psoralen and isopsoralen contribute to anticancer effect of P. corylifolia L.
ISSN:1741-427X
1741-4288
DOI:10.1093/ecam/nen087