A Dual Platform for Selective Analyte Enrichment and Ionization in Mass Spectrometry Using Aptamer-Conjugated Graphene Oxide

This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine and adenosine can be selectively enriched from plasma samples and that direct mass spectrometric readouts can b...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2010-12, Vol.132 (49), p.17408-17410
Main Authors: Gulbakan, Basri, Yasun, Emir, Shukoor, M. Ibrahim, Zhu, Zhi, You, Mingxu, Tan, Xiaohong, Sanchez, Hernan, Powell, David H, Dai, Hongjie, Tan, Weihong
Format: Article
Language:English
Subjects:
Adenosine - blood
Adenosine - isolation & purification
Aptamers, Nucleotide - chemistry
Cocaine - blood
Cocaine - isolation & purification
Graphite - chemistry
Humans
Mass Spectrometry - methods
Oxides - chemistry
Signal-To-Noise Ratio
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Summary:This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine and adenosine can be selectively enriched from plasma samples and that direct mass spectrometric readouts can be obtained without a matrix and with greatly improved signal-to-noise ratios. Aptamer-conjugated graphene oxide has clear advantages in target enrichment and in generating highly efficient ionization of target molecules for mass spectrometry. These results demonstrate the utility of the approach for analysis of small molecules in real biological samples.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja109042w