Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention

• Published in: Evidence-based complementary and alternative medicine 2011-01, Vol.2011 (2011), p.1-7
• Main Authors: Soliman, Sherry, Aronson, William J., Barnard, R. James
• Format: Article
• Language: English
• Subjects: Androgens; Apoptosis; Cell culture; Developmental stages; Diet; Exercise; Gene expression; High fat diet; Insulin; Insulin-like growth factor I; Insulin-like growth factors; Kinases; Lifestyles; Low fat diet; Medical research; NF-κB protein; Nutrient deficiency; Nutrition research; Oils & fats; Original; p53 Protein; Physical fitness; Prostate cancer; Proteins; Tumor cell lines
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1093/ecam/nep031

Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for these anticancer effects. Again, the intervention slowed growth and increased apoptosis in LNCaP cells; responses that were eliminated when IGF-I was added back to the post-intervention samples. The p53 protein content was increased and NFκB activation reduced in the post serum-stimulated LNCaP cells. Similar results were observed when the IGF-I receptor was blocked in the pre-intervention serum. In androgen-independent PC-3 cells, growth was reduced while none of the other factors were changed by the intervention. We conclude that diet and exercise intervention might help prevent clinical PCa as well as aid in the treatment of PCa during the early stages of development.