Role of pirenoxine in the effects of catalin on in vitro ultraviolet-induced lens protein turbidity and selenite-induced cataractogenesis in vivo

• Published in: Molecular vision 2011-07, Vol.17, p.1862-1870
• Main Authors: Hu, Chao-Chien, Liao, Jiahn-Haur, Hsu, Kuang-Yang, Lin, I-Lin, Tsai, Ming-Hsuan, Wu, Wen-Hsin, Wei, Tzu-Tang, Huang, Yi-Shiang, Chiu, Shih-Jiuan, Chen, Hsiang-Yin, Wu, Shih-Hsiung, Wu, Tzu-Hua
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Calcium - metabolism; Calcium - pharmacology; Calpain - adverse effects; Calpain - pharmacology; Cataract; Cataract - chemically induced; Cataract - drug therapy; Cataract - metabolism; Cataract - prevention & control; Chemistry; Chimie; Crystallin; Dose-Response Relationship, Drug; Drug Dosage Calculations; Electrophoresis, Polyacrylamide Gel; gamma-Crystallins - chemistry; gamma-Crystallins - metabolism; Human health sciences; Lens, Crystalline - drug effects; Lens, Crystalline - metabolism; Lens, Crystalline - pathology; Life sciences; Ophthalmic Solutions - administration & dosage; Ophthalmic Solutions - therapeutic use; Oxazines - administration & dosage; Oxazines - therapeutic use; Pharmacie, pharmacologie & toxicologie; Pharmacy, pharmacology & toxicology; Physical, chemical, mathematical & earth Sciences; Physique, chimie, mathématiques & sciences de la terre; Pirenoxine; Proteolysis - drug effects; Rats; Rats, Sprague-Dawley; Sciences de la santé humaine; Sciences du vivant; Sodium Selenite - administration & dosage; Sodium Selenite - adverse effects; Spectrum Analysis; Swine; Ultraviolet Rays
• ISSN: 1090-0535; 1090-0535

In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p