Development of a Semi-Field System for Contained Field Trials with Aedes aegypti in Southern Mexico

Development of new genetic approaches to either interfere with the ability of mosquitoes to transmit dengue virus or to reduce vector population density requires progressive evaluation from the laboratory to contained field trials, before open field release. Trials in contained outdoor facilities ar...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2011-08, Vol.85 (2), p.248-256
Main Authors: FACCHINELLI, Luca, VALERIO, Laura, GUILLERMO BOND, J, WISE DE VALDEZ, Megan R, HARRINGTON, Laura C, RAMSEY, Janine M, CASAS-MARTINEZ, M, SCOTT, Thomas W
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes - physiology
Aedes aegypti
Animals
Biological and medical sciences
Dengue virus
Female
Humidity
Infectious diseases
Male
Medical sciences
Mosquito Control - methods
Pest Control, Biological - methods
Population Dynamics
Reproduction - physiology
Temperature
Time Factors
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Summary:Development of new genetic approaches to either interfere with the ability of mosquitoes to transmit dengue virus or to reduce vector population density requires progressive evaluation from the laboratory to contained field trials, before open field release. Trials in contained outdoor facilities are an important part of this process because they can be used to evaluate the effectiveness and reliability of modified strains in settings that include natural environmental variations without releasing mosquitoes into the open field. We describe a simple and cost-effective semi-field system designed to study Aedes aegypti carrying a dominant lethal gene (fsRIDL) in semi-field conditions. We provide a protocol for establishing, maintaining, and monitoring stable Ae. aegypti population densities inside field cages.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2011.10-0426