Development and Validation of an Anodic Stripping Voltammetric Method for Determination of Zn2+ Ions in Brain Microdialysate Samples

An easy, rapid, and sensitive anodic stripping voltammetric method with a controlled growth mercury drop electrode has been developed and validated for the determination of Zn2+ ions in brain microdialysate samples obtained from rats. The considered level of the zinc concentration in the dialysate w...

Full description

Saved in:
Bibliographic Details
Published in:Biological trace element research 2011-09, Vol.142 (3), p.671-682
Main Authors: Opoka, Włodzimierz, Jakubowska, Małgorzata, Baś, Bogusław, Sowa-Kućma, Magdalena
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
brain
correlation
detection limit
electrolytes
ions
Life Sciences
mercury
Nutrition
Oncology
rats
zinc
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An easy, rapid, and sensitive anodic stripping voltammetric method with a controlled growth mercury drop electrode has been developed and validated for the determination of Zn2+ ions in brain microdialysate samples obtained from rats. The considered level of the zinc concentration in the dialysate was 0.5–6 ppb. In the investigated method, the stripping step was carried out by using a differential pulse potential-time voltammetric excitation signal. The optimal experimental conditions as well as the instrumental and accumulation parameters and supporting electrolyte composition were investigated. The optimized method was validated for precision, linearity, and accuracy. Mean recovery 82–110% was achieved, the precision expressed by CV not greater than 7.6% and the linearity given by correlation coefficient not lower than 0.9988. The limit of detection was 0.1 ppb. No interferences were observed. Due to high linearity, precision, and sensitivity, the developed method may be successfully applied in the determination of zinc ions in microdialysate brain samples. The results obtained for the first time demonstrate detailed characteristics of the determination of zinc in the brain microdialysate fluid by the ASV method. It may be applied in a wide range of physiological and pharmacological studies which focus on very low zinc concentration/alteration in various compartments of the organisms.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-010-8790-2