Apolipoprotein C-III and the Metabolic Basis for Hypertriglyceridemia and the Dense Low-Density Lipoprotein Phenotype

• Published in: Circulation (New York, N.Y.) N.Y.), 2010-04, Vol.121 (15), p.1722-1734
• Main Authors: CHUNYU ZHENG, KHOO, Christina, FURTADO, Jeremy, SACKS, Frank M
• Format: Article
• Language: English
• Subjects: Adult; Apolipoprotein C-III - metabolism; Apolipoproteins B - metabolism; Apolipoproteins E - metabolism; Biological and medical sciences; Blood and lymphatic vessels; Blood. Blood coagulation. Reticuloendothelial system; Cardiology. Vascular system; Cholesterol, VLDL - metabolism; Chromatography, Affinity; Dietary Carbohydrates - administration & dosage; Dietary Fats - administration & dosage; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Female; Humans; Hypertriglyceridemia - metabolism; Leucine - pharmacokinetics; Lipoproteins, LDL - biosynthesis; Lipoproteins, LDL - metabolism; Liver - metabolism; Male; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Phenotype; Phenylalanine - pharmacokinetics; Triglycerides - metabolism
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circulationaha.109.875807

Here, we aim to identify defects of apolipoprotein (apo) B lipoprotein metabolism that characterize hypertriglyceridemia, focusing on apoC-III and apoE. We studied the transport of plasma apoB within 21 distinct subfractions as separated by anti-apoC-III and anti-apoE immunoaffinity chromatography and ultracentrifugation in 9 patients with moderate hypertriglyceridemia and 12 normotriglyceridemic control subjects. Hypertriglyceridemia was characterized by a 3-fold higher liver secretion of very low-density lipoprotein (VLDL) that had apoC-III but not apoE and a 50% lower secretion of VLDL with both apoC-III and apoE (both P