Expression of cathepsin D and E-cadherin in primary laryngeal cancers correlation with neck lymph node involvement

• Published in: Journal of cancer research and clinical oncology 2011-09, Vol.137 (9), p.1371-1377
• Main Authors: Paksoy, Mustafa, Hardal, Umit, Caglar, Cakır
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antineoplastic agents; Biological and medical sciences; Biomarkers; Biomarkers, Tumor - metabolism; Cadherins - metabolism; Cancer Research; Carcinoma, Squamous Cell - diagnosis; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cathepsin D - metabolism; Female; Gene Expression Regulation, Neoplastic; Hematologic and hematopoietic diseases; Hematology; Humans; Internal Medicine; Laryngeal cancer; Laryngeal Neoplasms - diagnosis; Laryngeal Neoplasms - metabolism; Laryngeal Neoplasms - pathology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymph Nodes; Lymphatic Metastasis; Lymphatic system; Male; Medical sciences; Medicine; Medicine & Public Health; Metastasis; Middle Aged; Neck; Oncology; Original Paper; Otorhinolaryngology. Stomatology; Pharmacology. Drug treatments; Prognosis; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-011-1007-z

Objective The aim of this study was to investigate the relation between Cathepsin D (Cath-D) and E-Cadherin (E-Cad) expression levels in tumor tissue and neck lymph node metastasis. This attempt should be made to identify new factors that one could be useful in predicting clinical behavior. Study design In this study, we investigated rates of dying for E-Cad and Cath-D in paraphin blocks of larynx and neck dissection specimens taken from 56 selected patients. Laryngeal specimens dyed by ECad and Cath-D immunohistochemically. Immunoreactivity classified for E-Cad as positive (75% and above), reduced (25–75%) and negative (below 25%). Immunoreactivity classified for Cath-D:dye absorption rates higher than 50% evaluated positive and absorption rates lower than 50% evaluated negative. We compared the rates of dying for E-Cad and Cath-D groups to with or without neck lymph node metastasis in specimens. Results Neck metastasis were found in 41% of patients, in 55.6% of patients, if the E-Cad was negative, 40.6% if it was reduced, 33.3% if it was positive in specimens. The neck metastasis was found in 35.4% of patients with Cath-D expression negative, while 75% of patients was found with Cath-D expression positive. Conclusion Neck metastasis was found higher in E-Cad reduced group than positive group. Neck node metastasis was significantly higher in Cath-D positive group than Cath-D negative group. Cath-D and E-Cad essays may useful in identifying neck lymph node involvement. Cath-D expression levels are more in this predictive factor than E-Cad levels for possibility of neck lymph node metastasis in LSCC.