Specific detection of Salmonella enterica and Escherichia coli strains by using ELISA with bacteriophages as recognition agents

The use of bacteriophages, instead of antibodies, in the ELISA-based detection of bacterial strains was tested. This procedure appeared to be efficient, and specific strains of Salmonella enterica and Escherichia coli could be detected. The sensitivity of the assay was about 10 5 bacterial cells/wel...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2011-09, Vol.30 (9), p.1067-1073
Main Authors: Galikowska, E., Kunikowska, D., Tokarska-Pietrzak, E., Dziadziuszko, H., Łoś, J. M., Golec, P., Węgrzyn, G., Łoś, M.
Format: Article
Language:English
Subjects:
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteriological Techniques - methods
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
E coli
Enzyme-Linked Immunosorbent Assay - methods
Escherichia coli
Escherichia coli - isolation & purification
Escherichia coli - virology
Escherichia coli Infections - diagnosis
Human bacterial diseases
Humans
Infectious diseases
Internal Medicine
Medical Microbiology
Medical sciences
Salmonella
Salmonella enterica
Salmonella enterica - isolation & purification
Salmonella enterica - virology
Salmonella Infections - diagnosis
Salmonella Phages - growth & development
Sensitivity and Specificity
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Summary:The use of bacteriophages, instead of antibodies, in the ELISA-based detection of bacterial strains was tested. This procedure appeared to be efficient, and specific strains of Salmonella enterica and Escherichia coli could be detected. The sensitivity of the assay was about 10 5 bacterial cells/well (10 6 /ml), which is comparable with or outperforms other ELISA tests detecting intact bacterial cells without an enrichment step. The specificity of the assay depends on the kind of bacteriophage used. We conclude that the use of bacteriophages in the detection and identification of bacteria by an ELISA-based method can be an alternative to the use of specific antibodies. The advantages of the use of bacteriophages are their environmental abundance (and, thus, a possibility to isolate various phages with different specificities) and the availability of methods for obtaining large amounts of phage lysates, which are simple, rapid, cheap, and easy.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-011-1193-2