Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and s...

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Bibliographic Details
Published in:The Journal of infectious diseases 2011-09, Vol.204 (6), p.937-941
Main Authors: Kobayashi, Scott D., Malachowa, Natalia, Whitney, Adeline R., Braughton, Kevin R., Gardner, Donald J., Long, Dan, Wardenburg, Juliane Bubeck, Schneewind, Olaf, Otto, Michael, DeLeo, Frank R.
Format: Article
Language:English
Subjects:
Abscess - microbiology
Abscess - pathology
Abscesses
Animal models
Animals
BACTERIA
Bacterial Load
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Toxins - genetics
Bacterial Toxins - metabolism
Biological and medical sciences
Disease models
Disease Models, Animal
Exotoxins - genetics
Exotoxins - metabolism
Female
Fundamental and applied biological sciences. Psychology
Hemolysin Proteins - genetics
Hemolysin Proteins - metabolism
Histocytochemistry
Infections
Infectious diseases
Inoculation
Leukocidins - genetics
Leukocidins - metabolism
Major and Brief Reports
Medical sciences
Methicillin-Resistant Staphylococcus aureus - pathogenicity
Microbiology
Microscopy
Neutrophils
Rabbits
Skin - microbiology
Skin - pathology
Soft Tissue Infections - microbiology
Soft Tissue Infections - pathology
Staphylococcal Skin Infections - microbiology
Staphylococcal Skin Infections - pathology
Staphylococcus aureus
Toxins
Trans-Activators - genetics
Trans-Activators - metabolism
Virulence
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jir441