Recent advances in gene manipulation and nicotinic acetylcholine receptor biology

Pharmacological and immunological methods have been valuable for both identifying some native nicotinic acetylcholine receptor (nAChR) subtypes that exist in vivo and determining the neurobiological and behavioral role of certain nAChR subtypes. However, these approaches suffer from shortage of subt...

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Bibliographic Details
Published in:Biochemical pharmacology 2011-10, Vol.82 (8), p.808-819
Main Authors: Tammimäki, Anne, Horton, William J., Stitzel, Jerry A.
Format: Article
Language:English
Subjects:
acetylcholine
Acetylcholine receptors (nicotinic)
Animals
Assembly partners
Biological and medical sciences
Brain
Brain - metabolism
Drug Discovery - methods
Drug Discovery - trends
Gene manipulation
Gene Targeting - methods
Gene Targeting - trends
genes
Humans
Knock-in
Lentivirus
Localization
Medical sciences
Mice
Nervous system
neurophysiology
Nicotinic acetylcholine receptors
Pharmacology. Drug treatments
Protein Subunits
Receptors, Nicotinic - chemistry
Receptors, Nicotinic - genetics
Receptors, Nicotinic - physiology
Reviews
Targeted re-expression
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Summary:Pharmacological and immunological methods have been valuable for both identifying some native nicotinic acetylcholine receptor (nAChR) subtypes that exist in vivo and determining the neurobiological and behavioral role of certain nAChR subtypes. However, these approaches suffer from shortage of subtype specific ligands and reliable immunological reagents. Consequently, genetic approaches have been developed to complement earlier approaches to identify native nAChR subtypes and to assess the contribution of nAChRs to brain function and behavior. In this review we describe how assembly partners, knock-in mice and targeted lentiviral re-expression of genes have been utilized to improve our understanding of nAChR neurobiology. In addition, we summarize emerging genetic tools in nAChR research.
ISSN:0006-2952
1873-2968
DOI:10.1016/j.bcp.2011.06.014