Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning

We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of...

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Bibliographic Details
Published in:Genes & development 1998-04, Vol.12 (7), p.1036-1045
Main Authors: Niki, H, Hiraga, S
Format: Article
Language:English
Subjects:
Cell Cycle
Chromosomes - physiology
DNA, Bacterial - physiology
Escherichia coli
Escherichia coli - cytology
Escherichia coli - physiology
In Situ Hybridization, Fluorescence
Replication Origin - physiology
Research Paper
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Summary:We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC migrates rapidly to the opposite nucleoid border. These oriC copies are retained at both nucleoid borders, remaining at a constant distance from each cell pole. The terminus segment migrates from the nucleoid border to midcell and is retained there until the terminus is duplicated. The origin, terminus and other DNA regions show three migration patterns during active partitioning of daughter chromosomes.
ISSN:0890-9369
DOI:10.1101/gad.12.7.1036