Groucho acts as a corepressor for a subset of negative regulators, including Hairy and Engrailed

Relatively little is known about the molecular mechanisms involved in transcriptional repression, despite its importance in development and differentiation. Recent evidence suggests that some transcriptional repressors act by way of adaptor molecules known as corepressors. Here, we use in vivo funct...

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Bibliographic Details
Published in:Genes & development 1997-11, Vol.11 (22), p.3072-3082
Main Authors: Jiménez, G, Paroush, Z, Ish-Horowicz, D
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins
Basic Helix-Loop-Helix Transcription Factors
DNA-Binding Proteins - physiology
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila Proteins
Gene Expression Regulation, Developmental
Helix-Loop-Helix Motifs
Homeodomain Proteins - physiology
Insect Proteins - physiology
Macromolecular Substances
Protein Binding
Recombinant Fusion Proteins
Repressor Proteins - physiology
Research Paper
RNA-Binding Proteins - genetics
Transcription Factors
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Summary:Relatively little is known about the molecular mechanisms involved in transcriptional repression, despite its importance in development and differentiation. Recent evidence suggests that some transcriptional repressors act by way of adaptor molecules known as corepressors. Here, we use in vivo functional assays to test whether different repressor activities are mediated by the Groucho (Gro) corepressor in the Drosophila embryo. Previously, Gro was proposed to mediate repression by the Hairy-related family of basic helix-loop-helix proteins. Our results indicate not only that repression by Hairy requires Gro, but that a repressor domain from the Engrailed (En) homeodomain protein is also Gro dependent. The latter result correlates with an ability of this En domain to bind to Gro in vitro. In contrast, repressor regions from the Even-skipped, Snail, Krüppel, and Knirps transcription factors are effective in the absence of Gro. These results show that Gro is not generally required for repression, but acts as a specific corepressor for a fraction of negative regulators, including Hairy and En.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.11.22.3072