Gene Composer in a structural genomics environment

The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino‐acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high‐throughput protei...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2011-09, Vol.67 (9), p.985-991
Main Authors: Lorimer, Don, Raymond, Amy, Mixon, Mark, Burgin, Alex, Staker, Bart, Stewart, Lance
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
ClustalW
Gene Composer
Genetic engineering
Genomics
H1N1
influenza
Laboratory Communications
Molecular Sequence Data
protein structure
Proteins
Proteins - chemistry
Proteins - genetics
RNA polymerase
Software
structural genomics
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Summary:The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino‐acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high‐throughput protein structure determination, a database software package, Gene Composer, has been developed which facilitates the information‐rich design of protein constructs and their underlying gene sequences. With its modular workflow design and numerous graphical user interfaces, Gene Composer enables researchers to perform all common bioinformatics steps used in modern structure‐guided protein engineering and synthetic gene engineering. An example of the structure determination of H1N1 RNA‐dependent RNA polymerase PB2 subunit is given.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309111027424