The Protein Maker: an automated system for high-throughput parallel purification

The Protein Maker is an automated purification system developed by Emerald BioSystems for high‐throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. T...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2011-09, Vol.67 (9), p.1015-1021
Main Authors: Smith, Eric R., Begley, Darren W., Anderson, Vanessa, Raymond, Amy C., Haffner, Taryn E., Robinson, John I., Edwards, Thomas E., Duncan, Natalie, Gerdts, Cory J., Mixon, Mark B., Nollert, Peter, Staker, Bart L., Stewart, Lance J.
Format: Article
Language:English
Subjects:
Antibodies
automation
Automation - instrumentation
Automation - methods
chromatography
Coccidioides - chemistry
Coccidioides - enzymology
high-throughput protein purification
Influenza A virus
influenza virus
Laboratory Communications
Protein Maker
SSGCID
Sterol 14-Demethylase - isolation & purification
Sterol 14-Demethylase - metabolism
structural genomics
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Description
Summary:The Protein Maker is an automated purification system developed by Emerald BioSystems for high‐throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C‐terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X‐rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high‐throughput protein‐purification applications.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309111028776