XRCC3 promotes homology-directed repair of DNA damage in mammalian cells

Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and intera...

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Bibliographic Details
Published in:Genes & development 1999-10, Vol.13 (20), p.2633-2638
Main Authors: Pierce, A J, Johnson, R D, Thompson, L H, Jasin, M
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cricetinae
DNA Damage
DNA Repair - physiology
DNA-Binding Proteins - deficiency
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Gene Conversion
Gene Expression
Genetic Complementation Test
Green Fluorescent Proteins
Humans
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Rad51 protein
Rad51 Recombinase
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombination, Genetic
Research Communication
XRCC3 protein
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Summary:Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and interacts with it. Using a novel fluorescence-based assay, we demonstrate here that error-free homology-directed repair of DNA double-strand breaks is decreased 25-fold in an XRCC3-deficient hamster cell line and can be restored to wild-type levels through XRCC3 expression. These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal.
ISSN:0890-9369
DOI:10.1101/gad.13.20.2633