Effect of medium/ω‐6 long chain triglyceride‐based emulsion on leucocyte death and inflammatory gene expression

• Published in: Clinical and experimental immunology 2011-09, Vol.165 (3), p.383-392
• Main Authors: Cury‐Boaventura, M. F., Gorjão, R., Martins de Lima, T., Fiamoncini, J., Godoy, A. B. P., Deschamphs, F. C., Soriano, F. G., Curi, R.
• Format: Article
• Language: English
• Subjects: Adult; Analytical, structural and metabolic biochemistry; Apoptosis - drug effects; Biological and medical sciences; Cell culture; Cell death; Cell Death - drug effects; Cell Survival - drug effects; Cholesterol; Cholesterol - blood; Decanoic Acids - blood; DNA Fragmentation; Down-Regulation - drug effects; Down-Regulation - genetics; Fat Emulsions, Intravenous - chemistry; Fat Emulsions, Intravenous - metabolism; Fat Emulsions, Intravenous - pharmacology; Fatty acid composition; Fatty acids; Fatty Acids, Nonesterified - analysis; Fatty Acids, Nonesterified - blood; Fatty Acids, Omega-6 - blood; Fatty Acids, Omega-6 - chemistry; Fatty Acids, Omega-6 - metabolism; Fatty Acids, Omega-6 - pharmacology; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene Expression Regulation - drug effects; Gene Expression Regulation - genetics; Humans; Immune response; Inflammation; Inflammation - genetics; Leukocyte Count; Leukocytes (neutrophilic); Leukocytes - cytology; Leukocytes - drug effects; Leukocytes - metabolism; Lipids; lymphocyte; Lymphocytes; Lymphocytes - drug effects; Lymphocytes - metabolism; Lymphocytes - pathology; Male; MCT/ω‐6 LCT‐based emulsion; Necrosis - chemically induced; Necrosis - pathology; neutrophil; Neutrophils - drug effects; Neutrophils - pathology; Oxidative stress; Palmitic Acids - blood; parenteral nutrition; Proteolysis; Stearic Acids - blood; Thiobarbituric Acid Reactive Substances - metabolism; Transcription; Translational Studies; Triglycerides; Triglycerides - blood; Triglycerides - chemistry; Triglycerides - metabolism; Triglycerides - pharmacology; Up-Regulation - drug effects; Up-Regulation - genetics; Young Adult
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.2011.04432.x

Summary Lipid emulsion (LE) containing medium/ω‐6 long chain triglyceride‐based emulsion (MCT/ω‐6 LCT LE) has been recommended in the place of ω‐6 LCT‐based emulsion to prevent impairment of immune function. The impact of MCT/ω‐6 LCT LE on lymphocyte and neutrophil death and expression of genes related to inflammation was investigated. Seven volunteers were recruited and infusion of MCT/ω‐6 LCT LE was performed for 6 h. Four volunteers received saline and no change was found. Blood samples were collected before, immediately afterwards and 18 h after LE infusion. Lymphocytes and neutrophils were studied immediately after isolation and after 24 and 48 h in culture. The following determinations were carried out: plasma‐free fatty acids, triacylglycerol and cholesterol concentrations, plasma fatty acid composition, neutral lipid accumulation in lymphocytes and neutrophils, signs of lymphocyte and neutrophil death and lymphocyte expression of genes related to inflammation. MCT/ω‐6 LCT LE induced lymphocyte and neutrophil death. The mechanism for MCT/ω‐6 LCT LE‐dependent induction of leucocyte death may involve changes in neutral lipid content and modulation of expression of genes related to cell death, proteolysis, cell signalling, inflammatory response, oxidative stress and transcription.