insertional mutagenesis programme with an enhancer trap for the identification and tagging of genes involved in abiotic stress tolerance in the tomato wild-related species Solanum pennellii

• Published in: Plant cell reports 2011-10, Vol.30 (10), p.1865-1879
• Main Authors: Atarés, Alejandro, Moyano, Elena, Morales, Belén, Schleicher, Peter, García-Abellán, José Osvaldo, Antón, Teresa, García-Sogo, Begoña, Perez-Martin, Fernando, Lozano, Rafael, Flores, Francisco Borja, Moreno, Vicente, del Carmen Bolarin, María, Pineda, Benito
• Format: Article
• Language: English
• Subjects: abiotic stress; Agriculture; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cell Biology; Crops; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Drought; Droughts; enhancer elements; Enhancers; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Plant; genes; Genes, Plant; High-Throughput Screening Assays; Horticultural crops; insertional mutagenesis; Life Sciences; Lycopersicon esculentum; Mutagenesis, Insertional - methods; mutants; Original Paper; Phenotype; Plant Biochemistry; Plant Sciences; Progeny; Salinity; Salinity effects; salt tolerance; Salt-Tolerant Plants - genetics; Salt-Tolerant Plants - physiology; Salts; Solanum; Solanum - genetics; Solanum - physiology; Solanum pennellii; Stomata; Stress; stress tolerance; Stress, Physiological; T-DNA; Tomatoes; transfer DNA; Transformation; Transformation, Genetic; Trichomes; uidA gene; vascular bundles
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-011-1094-y

Salinity and drought have a huge impact on agriculture since there are few areas free of these abiotic stresses and the problem continues to increase. In tomato, the most important horticultural crop worldwide, there are accessions of wild-related species with a high degree of tolerance to salinity and drought. Thus, the finding of insertional mutants with other tolerance levels could lead to the identification and tagging of key genes responsible for abiotic stress tolerance. To this end, we are performing an insertional mutagenesis programme with an enhancer trap in the tomato wild-related species Solanum pennellii. First, we developed an efficient transformation method which has allowed us to generate more than 2,000 T-DNA lines. Next, the collection of S. pennelli T0 lines has been screened in saline or drought conditions and several presumptive mutants have been selected for their salt and drought sensitivity. Moreover, T-DNA lines with expression of the reporter uidA gene in specific organs, such as vascular bundles, trichomes and stomata, which may play key roles in processes related to abiotic stress tolerance, have been identified. Finally, the growth of T-DNA lines in control conditions allowed us the identification of different development mutants. Taking into account that progenies from the lines are being obtained and that the collection of T-DNA lines is going to enlarge progressively due to the high transformation efficiency achieved, there are great possibilities for identifying key genes involved in different tolerance mechanisms to salinity and drought.