Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes

The state-of-the-art cultured podocyte is conditionally immortalized by expression of a temperature-sensitive mutant of the SV40 large-T antigen. These cultures proliferate at 33°C and differentiate at 37°C into arborized cells that more closely resemble in vivo podocytes. However, the degree of res...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2011-09, Vol.301 (3), p.F660-F671
Main Authors: Chittiprol, Seetharamaiah, Chen, Phylip, Petrovic-Djergovic, Danica, Eichler, Tad, Ransom, Richard F
Format: Article
Language:English
Subjects:
Animals
Antigens
Biomarkers - metabolism
Cell culture
Cell Death - drug effects
Cell Differentiation - physiology
Cell Line
Cell Movement - physiology
Cell Proliferation
Cells, Cultured
Doxorubicin - pharmacology
Humans
Intracellular Signaling Peptides and Proteins - metabolism
Male
Membrane Proteins - metabolism
Mice
Microfilament Proteins - metabolism
Models, Animal
Mutation
Podocytes - cytology
Podocytes - drug effects
Podocytes - metabolism
Proteins
Puromycin Aminonucleoside - pharmacology
Rats
Rats, Sprague-Dawley
Rodents
Species Specificity
WT1 Proteins - metabolism
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Summary:The state-of-the-art cultured podocyte is conditionally immortalized by expression of a temperature-sensitive mutant of the SV40 large-T antigen. These cultures proliferate at 33°C and differentiate at 37°C into arborized cells that more closely resemble in vivo podocytes. However, the degree of resemblance remains controversial. In this study, several parameters were measured in podocyte cell lines derived from mouse (JR, KE), human (MS), and rat (HK). In all lines, the quantities of NEPH1 and podocin proteins and NEPH1 and SYNPO mRNAs were comparable to glomeruli, while synaptopodin and nephrin proteins and NPHS1 and NPHS2 mRNAs were
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00234.2011