Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors

To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2011-09, Vol.108 (37), p.15414-15419
Main Authors: Beier, Kevin T, Saunders, Arpiar, Oldenburg, Ian A, Miyamichi, Kazunari, Akhtar, Nazia, Luo, Liqun, Whelan, Sean P. J, Sabatini, Bernardo, Cepko, Constance L
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
birds
Brain
Brain - virology
Cell culture
Central nervous system
Central Nervous System - cytology
Disease transmission
Electrophysiology
Eye - virology
Fusion protein
genes
Genetic Vectors - genetics
glycoprotein G
Glycoproteins
Infection
Infections
Information processing
Lymphocytic choriomeningitis virus
Mice
Neural networks
Neurons
Neurons - metabolism
Olfactory Pathways - metabolism
Olfactory Pathways - virology
Pyramidal cells
Rabies
Rabies virus
Receptors
Recombination, Genetic - genetics
Staining and Labeling
Synapses
Synapses - metabolism
tracer techniques
Tracers
Vesicular stomatitis Indiana virus
Vesicular stomatitis virus
Vesiculovirus
Vesiculovirus - genetics
Viruses
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Summary:To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other viruses. The G protein from lymphocytic choriomeningitis virus endowed VSV with the ability to spread transsynaptically, specifically in an anterograde direction, whereas the rabies virus glycoprotein gave a specifically retrograde transsynaptic pattern. The use of an avian G protein fusion allowed specific targeting of cells expressing an avian receptor, which allowed a demonstration of monosynaptic anterograde tracing from defined cells. Synaptic connectivity of pairs of virally labeled cells was demonstrated by using slice cultures and electrophysiology. In vivo infections of several areas in the mouse brain led to the predicted patterns of spread for anterograde or retrograde tracers.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1110854108