Avian tropomyosin gene expression

Sequence analysis of overlapping fragments from a quail genomic library has revealed a tropomyosin gene consisting of 13 exons spaced over about 18 kilobase pairs of DNA. Skeletal muscle and smooth muscle transcripts share the same 5' untranslated sequence and may initiate from the same promote...

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Bibliographic Details
Published in:Nucleic acids research 1989-03, Vol.17 (5), p.2099-2118
Main Authors: LINDQUESTER, G. J, FLACH, J. E, FLEENOR, D. E, HICKMAN, K. H, DEVLIN, R. B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cloning, Molecular
Coturnix
DNA - isolation & purification
Exons
Fundamental and applied biological sciences. Psychology
Gene expression
Genes
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Muscle, Smooth - analysis
Muscles - analysis
Quail - genetics
Sequence Homology, Nucleic Acid
Tropomyosin - genetics
Tropomyosin - isolation & purification
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Summary:Sequence analysis of overlapping fragments from a quail genomic library has revealed a tropomyosin gene consisting of 13 exons spaced over about 18 kilobase pairs of DNA. Skeletal muscle and smooth muscle transcripts share the same 5' untranslated sequence and may initiate from the same promoter. However, the regions encoding amino acids 39-80 and 258-284 are specific to each muscle type. The two sets of exons encoding these regions undergo mutually exclusive alternative splicing in a tissue-specific manner as determined by Northern blots and S1-nuclease protection. Similarly, the 3' ends of the transcripts are different in skeletal muscle and smooth muscle, and each contains two polyadenylation signals which appear to be utilized in vivo. The avian alpha-tropomyosin gene is not expressed in cardiac muscle. The sequence of the gene shows great homology with other muscle-specific tropomyosins and includes a region homologous to the amino terminus of nonmuscle tropomyosins.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.5.2099