An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis

Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2011-10, Vol.22 (10), p.1885-1890, Article s13361-011-0203-z
Main Authors: Amstalden van Hove, Erika R., Smith, Donald F., Fornai, Lara, Glunde, Kristine, Heeren, Ron M. A.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Analytical, structural and metabolic biochemistry
Animals
Application Note
Bioinformatics
Biological and medical sciences
Biotechnology
Brain Chemistry
Chemistry
Chemistry and Materials Science
Female
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Histocytological Preparation Techniques - methods
Imaging
Ions
Male
Mass spectrometry
Mice
Molecular Imaging - methods
Myocardium - chemistry
Organic Chemistry
Peptides
Peptides - chemistry
Proteins
Proteins - chemistry
Proteomics
Rats
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectroscopy
Surface Properties
Surface treatment
Washing
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Summary:Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging.
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-011-0203-z