Different grades of agarose affect electrophoretic migration of large DNA molecules

The relation between DNA size and mobility during Pulsed Field Gel Electrophoresis (PFGE) is complex. Chromosome mobility varies with switch interval and between the type of system used e.g. PFGE and Field Inversion Gel Electrophoresis (FIGE). Here the authors show that the choice of agarose is crit...

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Bibliographic Details
Published in:Nucleic acids research 1989-04, Vol.17 (8), p.3315-3315
Main Authors: UPCROFT, J. A, BOREHAM, P. F. L, UPCROFT, P
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
DNA
DNA - analysis
Dna, deoxyribonucleoproteins
Electrophoresis - methods
Electrophoresis, Agar Gel - methods
Fundamental and applied biological sciences. Psychology
gel electrophoresis
Molecular Weight
Nucleic acids
Sepharose - standards
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Summary:The relation between DNA size and mobility during Pulsed Field Gel Electrophoresis (PFGE) is complex. Chromosome mobility varies with switch interval and between the type of system used e.g. PFGE and Field Inversion Gel Electrophoresis (FIGE). Here the authors show that the choice of agarose is critical for the separation and comparison of chromosomes and large DNA molecules. Chromosomes of Giardia intestinalis and lambda DNA size market (Clontech, Rockville, Maine) are separated in three grades of 0.7% Seakem GTG, Seakem ME and SeaPlaque agarose (Marine Colloids) by FIGE.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.8.3315