Clonal analysis of the proliferation potential of human bone marrow mesenchymal stem cells as a function of potency

Human mesenchymal stem cells (MSCs) from bone marrow are a heterogeneous ensemble of progenitors and lineage‐committed cells, with a broad range of regenerative properties. Ex vivo expansion to produce sufficient quantities of MSCs is essential for most therapeutic applications. The present study re...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2011-11, Vol.108 (11), p.2716-2726
Main Authors: Russell, Katie C., Lacey, Michelle R., Gilliam, Jennifer K., Tucker, H. Alan, Phinney, Donald G., O'Connor, Kim C.
Format: Article
Language:English
Subjects:
Adipogenesis
Aging
Apoptosis
Biocompatibility
Biological and medical sciences
Biomedical materials
Biotechnology
Bone Marrow
Cell Proliferation
Cell Survival
Chondrogenesis
Cloning
Colonies
Culture
Enzyme kinetics
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Human
Humans
Industrial applications and implications. Economical aspects
mesenchymal stem cells
Mesenchymal Stromal Cells - physiology
Miscellaneous
Osteogenesis
potency
proliferation
senescence
Stem cells
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Summary:Human mesenchymal stem cells (MSCs) from bone marrow are a heterogeneous ensemble of progenitors and lineage‐committed cells, with a broad range of regenerative properties. Ex vivo expansion to produce sufficient quantities of MSCs is essential for most therapeutic applications. The present study resolves the relationship between proliferation potential of MSCs and their potency. Clonal analysis generated single‐cell derived colonies of MSCs that were classified according to their trilineage potential to exhibit adipo‐ (A), chondro‐ (C), and osteogenesis (O) as a measure of potency. Multipotent OAC clones were highly proliferative with colony‐forming efficiencies that ranged from 35% to 90%; whereas, O clones formed colonies with an efficiency of 5% or less (P 
ISSN:0006-3592
1097-0290
1097-0290
DOI:10.1002/bit.23193