Isolation and sequencing of HRS60dim1, a dimeric member of the HRS60-family of a tobacco DNA repeat

Recently, a BamHI family of a repetitive DNA sequence was found in the nuclear DNA of N. tabacum , comprising ca. 2% of tobacco genome. This DNA repeat denoted as HRS60 consists of 182-184 bp units organized in tandems. After Southern blot hybridization of HRS60 with BamHI digest of tobacco nuclear...

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Bibliographic Details
Published in:Nucleic acids research 1989-06, Vol.17 (11), p.4377-4377
Main Authors: MATYASEK, R, KOUKALOVA, B, REICH, J
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Multigene Family
Nicotiana - genetics
Nicotiana tabacum
Plants, Toxic
Repetitive Sequences, Nucleic Acid
Sequence Homology, Nucleic Acid
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Summary:Recently, a BamHI family of a repetitive DNA sequence was found in the nuclear DNA of N. tabacum , comprising ca. 2% of tobacco genome. This DNA repeat denoted as HRS60 consists of 182-184 bp units organized in tandems. After Southern blot hybridization of HRS60 with BamHI digest of tobacco nuclear DNA, a ladder of hybridization bands appeared, corresponding to multiples of the monomeric unit. The cause of this "ladder-like" hybridization pattern might be mutation or methylation or steric inhibition of some BamHI restriction sites. A dimeric member of this family was analysed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.11.4377