Effect of Novel Marine Nutraceuticals on IL-1α-Mediated TNF-α Release from UVB-Irradiated Human Melanocyte-Derived Cells

UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5β-scymnol and CO2-supercritical fluid extract (CO2-SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effect...

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Bibliographic Details
Published in:Oxidative medicine and cellular longevity 2011-01, Vol.2011 (2011), p.1-11
Main Authors: Muthusamy, Visalini, Hodges, Lynn D., Macrides, Theodore A., Boyle, Glen M., Piva, Terrence J.
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Biphenyl Compounds
Cell Survival - drug effects
Cells, Cultured
Dietary Supplements
Humans
Hydrogen Peroxide
Inflammation
Interleukin-1alpha - immunology
Interleukin-1alpha - pharmacology
Iron
Melanocytes - cytology
Melanocytes - drug effects
Melanocytes - radiation effects
Melanocytes - secretion
Melanoma, Experimental - pathology
Mice
p38 Mitogen-Activated Protein Kinases - metabolism
Picrates
Reactive Oxygen Species - metabolism
Tumor Necrosis Factor-alpha - immunology
Tumor Necrosis Factor-alpha - secretion
Ultraviolet Rays
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Summary:UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5β-scymnol and CO2-supercritical fluid extract (CO2-SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor-α (TNF-α), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1α. Cell viability and TNF-α levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15 min after-irradiation. When α-tocopherol, CO2-SFE mussel oil, and 5β-scymnol were added to the UVB-irradiated HEM cells treated with IL-1α, TNF-α levels fell by 53%, 65%, and 76%, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes.
ISSN:1942-0900
1942-0994
DOI:10.1155/2011/728645