Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence

The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstrea...

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Bibliographic Details
Published in:Nucleic acids research 1989-07, Vol.17 (14), p.5501-5507
Main Authors: SPANJAARD, R. A, VAN DUIN, J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cloning, Molecular
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genes, Regulator
Interferon Type I - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
Peptide Chain Initiation, Translational
Plasmids
Protein Biosynthesis
Rats
RNA, Messenger - genetics
Transcription, Genetic
Translation. Translation factors. Protein processing
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Summary:The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstream gene by two to three orders of magnitude. This large difference allowed us to quantitate restarts in a meaningful manner. In the absence of a Shine and Dalgarno (SD) region reinitiation occurred but its efficiency was about 10% of that found in the SD carrying counterpart. We discuss three ways by which translational coupling between neighboring cistrons can be enforced.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.14.5501