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Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence

The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstrea...

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Published in:	Nucleic acids research 1989-07, Vol.17 (14), p.5501-5507
Main Authors:	SPANJAARD, R. A, VAN DUIN, J
Format:	Article
Language:	English
Subjects:	Animals Base Sequence Biological and medical sciences Cloning, Molecular Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genes, Regulator Interferon Type I - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation Peptide Chain Initiation, Translational Plasmids Protein Biosynthesis Rats RNA, Messenger - genetics Transcription, Genetic Translation. Translation factors. Protein processing
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container_issue	14
container_start_page	5501
container_title	Nucleic acids research
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creator	SPANJAARD, R. A VAN DUIN, J
description	The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstream gene by two to three orders of magnitude. This large difference allowed us to quantitate restarts in a meaningful manner. In the absence of a Shine and Dalgarno (SD) region reinitiation occurred but its efficiency was about 10% of that found in the SD carrying counterpart. We discuss three ways by which translational coupling between neighboring cistrons can be enforced.
doi_str_mv	10.1093/nar/17.14.5501
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A</creatorcontrib><creatorcontrib>VAN DUIN, J</creatorcontrib><title>Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstream gene by two to three orders of magnitude. This large difference allowed us to quantitate restarts in a meaningful manner. In the absence of a Shine and Dalgarno (SD) region reinitiation occurred but its efficiency was about 10% of that found in the SD carrying counterpart. We discuss three ways by which translational coupling between neighboring cistrons can be enforced.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Interferon Type I - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Peptide Chain Initiation, Translational</subject><subject>Plasmids</subject><subject>Protein Biosynthesis</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Translation. 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Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Interferon Type I - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Peptide Chain Initiation, Translational</topic><topic>Plasmids</topic><topic>Protein Biosynthesis</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Translation. Translation factors. Protein processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SPANJAARD, R. 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source	Open Access: PubMed Central; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects	Animals Base Sequence Biological and medical sciences Cloning, Molecular Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genes, Regulator Interferon Type I - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation Peptide Chain Initiation, Translational Plasmids Protein Biosynthesis Rats RNA, Messenger - genetics Transcription, Genetic Translation. Translation factors. Protein processing
title	Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence
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